研究方向：1. T细胞免疫在流感/新冠感染中的机制及应用研究2. T细胞TCR多样性与对病毒交叉反应性的关系研究3. 骨髓移植后CMV/EBV复发的T细胞治疗与预后诊断研究；

研究工作：1.解析 T 细胞免疫在急性病毒（流感、新冠）和潜伏病毒（CMV、EBV）感染中的作用机制。（1）研究感染中驱动病毒特异性 T 细胞激活、克隆扩增、TCR 多样性和 T细胞耗竭的分子调控网络，揭示流感（如 H7N9）或新冠感染 T 细胞激活和衰竭的免疫机理。（2）研究 CMV/EBV 感染中病毒特异性 T 细胞与血浆 DNA 的动力学关系，以及 CMV T 细胞记忆通胀（memory inflation）的机制。

2. 高效筛选 T 细胞表位和高功能性 TCR-T，推动复发难治性 CMV 和 EBV相关肿瘤 T 细胞治疗与预后诊断，构建通用型广谱 T 细胞疫苗研发。（1）针对特定 HLA，描绘 T 细胞抗原图谱和主导表位 T 细胞的 TCR，制备筛选用的四聚体和 TCR-T，探索特异性 CMV/EBV T 细胞治疗。（2）结合数据库预测获得的新冠、流感 T 细胞表位，开展 T 细胞表位串联或者多条多肽构建T 细胞疫苗，应用乳酸杆菌载体构建口服通用型流感 T 细胞疫苗。

代表性科研项目：

1. 同科同属病毒通用T 细胞免疫特征及免疫原筛选 国家重点研发计划 2022.12-2025.11

2. 口岸致灾因子关键技术研究及装备研制 国家重点研发计划 2020.01-2022.12

3. 新冠病毒特异性T细胞反应特征及其对中和抗体长期维持机制研究 国家自然科学基金面上项目 2023.01-2026.12

4. 严重流感感染中人体CD8+ T细胞免疫激活和免疫衰竭的机制研究 国家自然科学基金面上项目 2020.01-2023.12

5. H7N9感染时机体的细胞免疫应答及其保护作用研究 国家自然科学基金面上项目 2015.01-2018.12

6. 禽流感和中东呼吸综合征免疫致病机制研究及其疫苗和抗体药物研发 广东省重点项目 2020.07-2022.12

7. 新冠病毒突破感染机制与序贯免疫保护机制研究 广东省重点项目 2022.07-2024.12

代表性学术论文（参考文献格式）：

1. Wang J, Li K, Mei X, Cao J, Zhong J, Huang P, Luo Q, Li G, Wei R, Zhong N, Zhao Z, Wang Z. SARS-CoV-2 vaccination-infection pattern imprints and diversifies T cell differentiation and neutralizing response against Omicron subvariants. Cell Discov. 2022 Dec 21;8(1):136. doi: 10.1038/s41421-022-00501-3. PMID: 36543767; PMCID: PMC9769462

2. He L, Zhong J, Li G, Lin Z, Zhao P, Yang C, Wang H, Zhang Y, Yang X, Wang Z. Development of SARS-CoV-2 animal vaccines using a stable and efficient NDV expression system. J Med Virol. 2023 Jan;95(1):e28237. doi: 10.1002/jmv.28237. Epub 2022 Oct 28. PMID: 36258299; PMCID: PMC9874532.

3. Zhang, Jing, Jinpeng Cao, Runhui Zheng, M. D. Yu, Zhengfang Lin, Caixia Wang, James McCluskey, Ji Yang, Zhenjun Chen, Alexandra J. Corbett, Pengxing Cao, Wen-jian Mo and Zhongfang Wang. “The Establishment of a CMV-Specific CD8 + T-Cell Threshold by Kinetic Modelling for Prediction of Post-HSCT Reactivation.” SSRN Electronic Journal (2022): n. pag.

4. Zhong J, Liu S, Cui T, Li J, Zhu F, Zhong N, Huang W, Zhao Z, Wang Z. Heterologous booster with inhaled adenovirus vector COVID-19 vaccine generated more neutralizing antibodies against different SARS-CoV-2 variants. Emerg Microbes Infect. 2022 Dec;11(1):2689-2697. doi: 10.1080/22221751.2022.2132881. PMID: 36197655; PMCID: PMC9639505.

5. Xiaoqiang Liu, Yuhua Li, Zhongfang Wang, Shouchun Cao, Weijin Huang, Lin Yuan, Yi-Jiao Huang, Yan Zheng, Jingjing Chen, Bo Ying, Zuoyun Xiang, Jin Shi, Jincun Zhao , Zhen Huang and Cheng-Feng Qin Safety and superior immunogenicity of heterologous boosting with an RBD-based SARS-CoV-2 mRNA vaccine in Chinese adults Cell Research (2022) 32:777–780(共一第三)

6. Wang Z, Yang X, Mei X, Zhou Y, Tang Z, Li G, Zhong J, Yu M, Huang M, Su X, Lin B, Cao P, Yang J, Ran P. SARS-CoV-2-specific CD4+ T cells are associated with long-term persistence of neutralizing antibodies. Signal Transduct Target Ther. 2022 Apr 23;7(1):132. doi: 10.1038/s41392-022-00978-0. PMID: 35461307; PMCID: PMC9034077.

7. Wang Z, Zhao Z, Cui T, Huang M, Liu S, Su X, Li G, Song T, Li W, Zhong N, Xu M, Yang X, Huang W. Heterologous boosting with third dose of coronavirus disease recombinant subunit vaccine increases neutralizing antibodies and T cell immunity against different severe acute respiratory syndrome coronavirus 2 variants. Emerg Microbes Infect. 2022 Dec;11(1):829-840. doi: 10.1080/22221751.2022.2048969. PMID: 35230230; PMCID: PMC8928863.

8. Wang Z, Yang X, Zhong J, Zhou Y, Tang Z, Zhou H, He J, Mei X, Tang Y, Lin B, Chen Z, McCluskey J, Yang J, Corbett AJ, Ran P. Exposure to SARS-CoV-2 generates T-cell memory in the absence of a detectable viral infection. Nat Commun. 2021 Mar 19;12(1):1724. doi: 10.1038/s41467-021-22036-z. PMID: 33741972; PMCID: PMC7979809.

9. Wang Z, Yang X, Zhou Y, Sun J, Liu X, Zhang J, Mei X, Zhong J, Zhao J, Ran P. COVID-19 Severity Correlates with Weaker T-Cell Immunity, Hypercytokinemia, and Lung Epithelium Injury. Am J Respir Crit Care Med. 2020 Aug 15;202(4):606-610.

10. Wang Z, Zhu L, Nguyen THO, Wan Y, Sant S, Quiñones-Parra SM, Crawford JC, Eltahla AA, Rizzetto S, Bull RA, Qiu C, Koutsakos M, Clemens EB, Loh L, Chen T, Liu L, Cao P, Ren Y, Kedzierski L, Kotsimbos T, McCaw JM, La Gruta NL, Turner SJ, Cheng AC, Luciani F, Zhang X, Doherty PC, Thomas PG, Xu J, Kedzierska K. Clonally diverse CD38+HLA-DR+CD8+ T cells persist during fatal H7N9 disease. Nat Commun. 2018 Feb 26;9(1):824.

11. Tingting Cui, Mingzhu Huang, Xiaoling Su, Zhengfang Lin, Jiaying Zhong, Xiaoyun Yang, Zhongfang Wang Potential of Antibody-Dependent Cellular Cytotoxicity in Acute and Recovery Phases of SARS-CoV-2 Infection. Infectious Diseases & Immunity 2022;2(2)

12. Huimeng Wang, Adam G Nelson, Bingjie Wang, Zhe Zhao, Xin Yi Lim, Mai Shi, Lucy J Meehan, Xiaoxiao Jia, Katherine Kedzierska, Bronwyn S Meehan, Sidonia BG Eckle, Michael NT Souter, Troi J Pediongco, Jeffrey YW Mak, David P Fairlie, James McCluskey, Zhongfang Wang, Alexandra J Corbett & Zhenjun Chen The balance of interleukin-12 and interleukin-23 determines the bias of MAIT1 versus MAIT17 responses during bacterial infection.  Immunology & Cell Biology 2022; 100: 547–561  2022.05.04

13. Weiping Qian , Shu Xia , Xiaoyun Yang , Jiaying Yu, Bingpeng Guo, Zhengfang Lin, Rui Wei, Mengmeng Mao, Ziyi Zhang , Gui Zhao, Junye Bai, Qian Han, Zhongfang Wang and Qun Luo Complex Involvement of the Extracellular Matrix, Immune Effect, and Lipid Metabolism in the Development of Idiopathic Pulmonary Fibrosis Frontiers in Molecular Biosciences January 2022 | Volume 8 | Article 800747

14. Xiaoxiao Jia, Brendon Y Chua, Liyen Loh, Marios Koutsakos, Lukasz Kedzierski,Moshe Olshansky, William R Heath, So Young Chang, Jianqing Xu, Zhongfang Wang, and Katherine Kedzierska corresponding author High expression of CD38 and MHC class II on CD8+ T cells during severe influenza disease reflects bystander activation and trogocytosis. Clin Transl Immunology. 2021.Sep.08 10(9): e1336 doi: 10.1002/cti2.1336

15. Wu F, Zhou Y, Wang Z#, Xie M, Shi Z, Tang Z, Li X, Li X, Lei C, Li Y, Ni Z, Hu Y, Liu X, Yin W, Cheng L, Ye F, Peng J, Huang L, Tian J, Zhang L, Mo X, Zhang Y, Hu K, Jiang Y, Guan W, Xiang J, Liu Y, Peng Y, Wei L, Hu Y, Peng P, Wang J, Liu J, Huang W, Chen R, Zhao J, Li S, Zhang N, Zhao J, Zhong N, Ran P; Medical Treatment Expert Group for COPD and COVID-19. Clinical characteristics of COVID-19 infection in chronic obstructive pulmonary disease: a multicenter, retrospective, observational study. Jouranl of Thoracic Disease. 2020 May;12(5):1811-1823.

获奖及个人荣誉：

1.省部级奖励 珠江学者 广东省教育厅

2.省部级奖励 高层次留学人才 人力资源和社会保障部

3.广州市高层次人才-优秀专家 A 证 广州市人才领导工作小组

4.南山学者优秀人才 广州医科大学

5.三级教授 广州医科大学